recombinant human integrins αvβ1, αvβ5 αvβ6  (R&D Systems)

Journal: bioRxiv

Article Title: Defining the mechanism of galectin-3-mediated TGF-β1 activation and its role in lung fibrosis

doi: 10.1101/2023.10.11.561855

Figure Lengend Snippet: Soluble galectin-3 (sequential injections, 19.5 - 5000 nM) binding to glycosylated or deglycosylated αv integrins: (A) αvβ1, (B) αvβS and (C) αvβ6 immobilised on the surface of a Series S sensor chip CM5 (approximately 1000 RU). (D) Soluble galectin-3 (sequential injections, 156.3-20000 nM) binding to glycosylated or deglycosylated TGFβRII immobilised to a Series S sensor chip CMS (approximately 400 RU). SPR signals were measured in RU and all sensorgrams baseline-corrected. Binding response values plotted in GraphPad Prism with connecting line/curve shown.

Article Snippet: Commercially available recombinant human integrins αvβ1, αvβS and αvβ6 (R&D Systems) or TGF beta receptor II protein (Abcam) were diluted in 10 mM sodium acetate pH 4.0 immobilisation buffer and immobilised onto a Series S Sensor Chip CM5 (Cytiva) via the standard amine coupling protocol.

Techniques: Binding Assay

Journal: bioRxiv

Article Title: Defining the mechanism of galectin-3-mediated TGF-β1 activation and its role in lung fibrosis

doi: 10.1101/2023.10.11.561855

Figure Lengend Snippet: Solution competition binding assays performed with the galectin-3 inhibitor GB0139 (blue) or GB1107 (black) for αv integrins: (A) αvβ1, (B) αvβS and (C) αvβ6 or (D) TGFβRII in the presence of galectin-3 at 625 nM. Response values are normalised with respect to the highest binding response (DMSO control) and competitive inhibition graphs plotted in GraphPad Prism. IC50 values were calculated by non-linear regression analysis (binding saturation) - specific binding with hill slope.

Article Snippet: Commercially available recombinant human integrins αvβ1, αvβS and αvβ6 (R&D Systems) or TGF beta receptor II protein (Abcam) were diluted in 10 mM sodium acetate pH 4.0 immobilisation buffer and immobilised onto a Series S Sensor Chip CM5 (Cytiva) via the standard amine coupling protocol.

Techniques: Binding Assay, Inhibition

Journal: The Journal of biological chemistry

Article Title: Receptor-binding domain of SARS-CoV-2 is a functional αv-integrin agonist.

doi: 10.1016/j.jbc.2023.102922

Figure Lengend Snippet: Figure 3. Recombinant human integrins αvβ3 and αvβ6, but not α5β1, bind to immobilized S1-RBD. Representative kinetic data for αvβ3 (A and D), αvβ6 (B and E), or α5β1 (C and F) binding to immobilized S1-RBD (A–C), FNIII10 (D–E), or FNIII8-10 (F). Data are presented as representative traces (bold colored lines) collected from one of two (α5β1, 100–1000 nM) or three (αvβ3, 5–450 nM and αvβ6, 5–500 nM) experiments and corresponding 1:1 binding fits (black lines).

Article Snippet: Recombinant human integrins αvβ3 (3050-AV), αvβ6 (3817- AV), and α5β1 (3230-A5) were from R&D Systems.

Techniques: Recombinant, Binding Assay